Assessing a New Tool for Early Detection of Endangered Turtles on Proposed Transportation Projects Status: CompleteReport Date: 07/17/2025 Summary: More than 60% of the world’s turtle species are endangered, including the Blanding’s and wood turtles native to Minnesota. In a recent study, researchers tested whether DNA from turtles could be reliably detected in water samples to indicate the presence of the species at a site. The results were clear: Environmental DNA (eDNA) surveys accurately and efficiently indicate the presence of turtle species at considerably lower cost than traditional trapping or visual surveys. eDNA technology will allow MnDOT to base decisions on real-time data, reducing unnecessary mitigation efforts and project delays while ensuring continued protection of vulnerable turtle populations and their habitats at transportation project sites. Further research is needed to fine-tune wood turtle detection. With broader use, eDNA could transform how Minnesota safeguards its rarest species—quickly, cost-effectively and confidently. Final Deliverables: Assessing a New Tool for Early Detection of Endangered Turtles on Proposed Transportation Projects (Report #2025-35) Detecting Endangered Turtles with Environmental DNA (Research Summary) Related Materials: Detecting Endangered Turtles with Environmental DNA (Blog Post) New Project: Assessing a New Tool for Early Detection of Endangered Turtles on Proposed Transportation Projects (Blog Post) Related Research: Reduce Vehicle-Animal Collisions with Installation of Small Animal Exclusion Fencing